B9673

1-Bromo-3-chloropropane

for isolation of RNA

• 동의어(들): 1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
• Linear Formula: Cl(CH₂)₃Br
• CAS Number: 109-70-6
• Molecular Weight: 157.44
• Beilstein: 605278
• EC Number: 203-697-1
• MDL number: MFCD00000998
• UNSPSC 코드: 41105515
• PubChem Substance ID: 24892149
• NACRES: NA.52
• Grade: for molecular biology
• 분석: ≥98.5% (GC)
• bp: 144-145 °C (lit.)

속성

• Grade: for molecular biology
• Quality Level: 200
• 분석: ≥98.5% (GC)
• 양식: liquid
• refractive index: n20/D 1.486 (lit.)
• bp: 144-145 °C (lit.)
• density: 1.592 g/mL at 25 °C (lit.)
• 저장 온도: room temp
• SMILES string: ClCCCBr
• InChI: 1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
• InChI key: MFESCIUQSIBMSM-UHFFFAOYSA-N

설명

일반 설명

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA.[1] It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

애플리케이션

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

• feline teeth[2]
• mice brain and liver tissues[3]
• human ectocervical epithelial cells[4]
• placental samples[4]
• human mesenchymal stromal cells (hMSCs)[5]

특징 및 장점

• less toxic alternative to chloroform
• compatible with TRI reagent for isolation of RNA with decreased DNA contamination

기타 정보

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

안전성 정보

• 픽토그램: GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H302,H331,H335,H341,H412
• 예방조치 성명서: P202 - P261 - P273 - P301 + P312 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter