T3934

TRI Reagent^®

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

• 동의어(들): DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, cell CSF amniotic fluid RNA extraction reagent, cell CSF amniotic fluid RNA isolation reagent, cell CSF amniotic fluid RNA purification reagent, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction solution, total RNA isolation solution, total RNA purification solution, TRI Reagent^® RNA Isolation Reagent
• MDL number: MFCD00213058
• UNSPSC 코드: 12352200
• NACRES: NA.52

속성

• Quality Level: 100
• 사용: 0.75 mL sufficient for 0.25 mL fluid samples

설명

일반 설명

TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.

A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.

TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.

This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

애플리케이션

TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

TRI Reagent^® has been used in RNA extraction.[1][2][3]

The reagent has been used for isolating total RNA.[4] The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.

특징 및 장점

• Easily scalable RNA isolation
• Works with many sources: human, plant, yeast, bacterial, or viral
• Better yields than traditional guanidine thiocyanate/cesium chloride methods

법적 정보

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

안전성 정보

• 픽토그램: GHS06,GHS08,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H301 + H311 + H331,H314,H341,H373,H411
• 예방조치 성명서: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• 표적 기관: Nervous system,Kidney,Liver,Skin
• 보충제 위험성: EUH032
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): 174.2 °F - closed cup
• Flash Point (°C): 79 °C - closed cup