R4533

RNAzol^® RT

For processing total and small RNA from human, animal, plant, bacterial,and viral samples

• 동의어(들): single step RNA isolation, single step RNA purification, total RNA isolation, total RNA purification
• UNSPSC 코드: 12352200
• NACRES: NA.52

속성

• Quality Level: 100
• 사용: mL sufficient for 10⁷ cells; mL sufficient for 100 mg tissue (or)

설명

일반 설명

RNAzol RT is a quick and convenient reagent for use in the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin.[1][2][3] A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol^® RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction.

This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol^® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.

This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.

애플리케이션

Isolated RNA can be used for Northern blots, RNase protection assay, microarrays, PCR, and other molecular biology applications.

RNAzol RT is a highly effective reagent for the single-step isolation of total and small RNA from human, animal, plant, bacterial and viral origin samples. The RNA isolation method based on this reagent is performed at room temperature, without the use of chloroform for phase separation, and allows for use in RT-PCR without requiring DNase treatment.

특징 및 장점

• Isolates micro RNA and total RNA isolation of pure and undegraded RNA from biological samples
• High yields and increased quality of isolated RNA
• Completed at room temperature in less than an hour starting with fresh tissue or cells

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

RNAZol is a registered trademark of Molecular Research Center, Inc.

안전성 정보

• 픽토그램: GHS08,GHS05,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302 + H312 + H332,H314,H341,H373,H411
• 예방조치 성명서: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• 표적 기관: Nervous system,Kidney,Liver,Skin
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point (°F): 230.0 °F
• Flash Point (°C): 110 °C