A6691
Amidase from Pseudomonas aeruginosa
recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)
동의어(들):
Acrylamide Amidohydrolase, Acylase
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250 UNITS
₩775,359
1000 UNITS
₩1,327,113
About This Item
CAS Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54
추천 제품
재조합
expressed in E. coli
양식
buffered aqueous glycerol solution
hydroxamate transferase activity
≥200 units/mg protein (biuret)
농도
14 mg/mL
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
Pseudomonas aeruginosa PAO1 ... PA4163(880181)
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애플리케이션
Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.[1]
The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater [2].
생화학적/생리학적 작용
The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment [2].
단위 정의
One unit will convert 1.0 μmole of acetamide and hydroxylamine to acetohydroxamate and ammonia per min at pH 7.2 at 37 °C.
물리적 형태
Solution in 50% glycerol containing 7 mM 2-mercaptoethanol and phosphate buffer salt
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Substrate interaction with recombinant amidase from Pseudomonas aeruginosa during biocatalysis
Pacheco R, Karmali A
Biocatalysis and Biotransformation, 27(5/6), 367-376 (2009)
Jorge Andrade et al.
The Journal of biological chemistry, 282(27), 19598-19605 (2007-04-20)
Microbial amidases belong to the thiol nitrilases family and have potential biotechnological applications in chemical and pharmaceutical industries as well as in bioremediation. The amidase from Pseudomonas aeruginosa isa6 x 38-kDa enzyme that catalyzes the hydrolysis of a small range
Butyramide-utilizing mutants of Pseudomonas aeruginosa 8602 which produce an amidase with altered substrate specificity.
J E Brown et al.
Journal of general microbiology, 57(2), 273-285 (1969-08-01)
Arthur J L Cooper et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 163(1), 108-120 (2012-05-23)
Many cancer cells have a strong requirement for glutamine. As an aid for understanding this phenomenon the (18)F-labeled 2S,4R stereoisomer of 4-fluoroglutamine [(2S,4R)4-FGln] was previously developed for in vivo positron emission tomography (PET). In the present work, comparative enzymological studies
Raffaella Gandolfi et al.
Bioorganic & medicinal chemistry letters, 22(16), 5283-5287 (2012-07-17)
The chemoenzymatic deacylation of ramoplanin A2 is described for the first time: ramoplanin A2 was Boc-protected and hydrogenated to Boc-protected tetrahydroramoplanin, which was subsequently deacylated using an acylase from Actinoplanes utahensis NRRL 12052. The chemoenzymatic process proceeded with 80% overall
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