추천 제품
생물학적 소스
Escherichia coli
형태
suspension
특이 활성도
5-10 units/mg protein
분자량
Mr ~70000
기술
activity assay: suitable
응용 분야
diagnostic assay manufacturing
저장 온도
2-8°C
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일반 설명
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.
애플리케이션
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics. It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp. Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.
생화학적/생리학적 작용
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression. It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.
단위 정의
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C
기타 정보
Characterization; In enantioselective resolution; Synthesis of ampicillin and benzylpenicillin
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Penicillin Acylase in the Industrial Production of ?-Lactam Antibiotics
Bruggink A, Roos EC, Vroom ED
Organic Process Research & Development, 2(2), 128-133 (1998)
A. Guy et al.
Bioorganic & Medicinal Chemistry Letters, 3, 1041-1041 (1993)
Lavinia Dunsmore et al.
Nature chemistry, 14(7), 754-765 (2022-06-29)
Natural products that contain ortho-quinones show great potential as anticancer agents but have been largely discarded from clinical development because their redox-cycling behaviour results in general systemic toxicity. Here we report conjugation of ortho-quinones to a carrier, which simultaneously masks
Ampicillin and cephalexin synthesis catalysed by E. coli penicillin amidase. Yield increase due to substrate recycling
Kasche, V
Biotechnology Letters, 7, 877-882 (1985)
Penicillin acylase (bacterial).
T A Savidge et al.
Methods in enzymology, 43, 705-721 (1975-01-01)
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