Potassium buffer is not required. For assay purposes, this product is reconstituted in potassium phosphate buffer, pH 6.0, at 0.2 mg/ml, Solutions may also be prepared 40 mM Tris HCL, pH 7.5, containing 50 mM NaCl and 50% glycerol. This stock may be stored for extended periods at –20 °C. Other sources indicate that this enzyme may also be prepared in water containing 0.01% BSA and TBE buffer. These options have not been validated. Please see the link below to review a publication that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817930/
A6306
Agarase from Pseudomonas atlantica
lyophilized powder, ≥5,000 units/mg protein (Lowry)
동의어(들):
β-Agarase, Agarose 4-glycanohydrolase
로그인조직 및 계약 가격 보기
크기 선택
1000 UNITS
₩419,398
5000 UNITS
₩1,489,208
₩419,398
예상 입고일2025년 3월 24일세부사항
모든 사진(2)
크기 선택
보기 변경
1000 UNITS
₩419,398
5000 UNITS
₩1,489,208
About This Item
CAS Number:
MDL number:
UNSPSC 코드:
12352204
eCl@ss:
42040102
NACRES:
NA.26
₩419,398
예상 입고일2025년 3월 24일세부사항
추천 제품
단위 정의
One unit will produce 1.0 μg of reducing sugar (measured as D-galactose) from agar per min at pH 6.0 at 40 °C.
물리적 형태
Product is formulated with phosphate buffered salts, bovine serum albumin and Agarase. Total protein content ranges between 10 – 30% w/w.
기타 정보
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Yuji Hatada et al.
Marine biotechnology (New York, N.Y.), 13(3), 411-422 (2010-08-06)
A gene of unknown function from the genome of the agar-degrading deep-sea bacterium Microbulbifer thermotolerans JAMB-A94(T) was functionally identified as a ι-carrageenase gene. This gene, designated as cgiA, is located together with two β-agarase genes, agaA and agaO in a
Agarolytic bacterium Persicobacter sp. CCB-QB2 exhibited
a diauxic growth involving galactose utilization pathway
a diauxic growth involving galactose utilization pathway
Go Furusawa
Microbiology (2016)
The effects of short- and long-term freezing on Porphyra umbilicalis Kutzing (Bangiales, Rhodophyta) blade viability
Lindsay Green
Journal of Embryology and Experimental Morphology (2014)
Uyangaa Temuujin et al.
Applied microbiology and biotechnology, 92(4), 749-759 (2011-06-10)
The DagA product of Streptomyces coelicolor is an agarase with a primary translation product (35 kDa) of 309 amino acids, including a 30-amino acid signal peptide. Although dagA expression in Streptomyces lividans under the control of its own set of
Seungwoo Lee et al.
Journal of microbiology and biotechnology, 21(11), 1116-1122 (2011-12-01)
In this study, site-directed mutagenesis was performed on the β-agarase AgaA gene from Zobellia galactanivorans to improve its catalytic activity and thermostability. The activities of three mutant enzymes, S63K, C253I, and S63K-C253I, were 126% (1,757.78 U/mg), 2.4% (33.47 U/mg), and
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Will this enzyme work in water or PBS, or must it be a potassium buffer (for which not further details are provided in the spec sheet)?
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