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A1602

Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

ammonium sulfate suspension, ≥40 units/mg protein

동의어(들):

1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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크기 선택

25 MG
₩137,837
100 MG
₩417,977

₩137,837

구입 가능 여부는 고객센터에 문의하십시오.
기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요
모든 사진(1)

크기 선택

보기 변경
25 MG
₩137,837
100 MG
₩417,977

About This Item

CAS Number:
9032-08-0
효소 위원회 번호:
3.2.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00081350
UNSPSC 코드:
12352204
NACRES:
NA.54

₩137,837

구입 가능 여부는 고객센터에 문의하십시오.
기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요

양식

ammonium sulfate suspension

특이 활성도

≥40 units/mg protein

배송 상태

wet ice

저장 온도

2-8°C

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관련 카테고리

일반 설명

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type.[1] It is produced by several species of Aspergillus genus.[2]

애플리케이션

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle [3].
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate.[4] It has also been used in digestive enzyme mixture for starch digestion.[5]

생화학적/생리학적 작용

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides [6].

단위 정의

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

물리적 형태

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000373075
0000373075
0000254985
0000254985
SLCL3569

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COA 검색

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문서

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

프로토콜

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

질문

1–2 / 2 질문  

  1. What volume of ammonium sulfate is the 25mg suspended in? 

    1 답변

    1. This product is filled by density, with a target fill volume of 0.5 ml for the 25mg and 2.0 ml for the 100mg package sizes.

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  2. How can a 1mg/mL solution of amyloglucosidase A1602 be prepared in acetate buffer?

    1 답변

    1. The product A1602 is in an ammonium sulfate solution. Here's how you can work with this product:

      - If the ammonium sulfate might interfere with the reaction(s):
      1. Remove the enzyme from the refrigerator, ensuring that the ammonium sulfate suspensions are not frozen.
      2. Gently invert the vial or bottle to produce a milky suspension. Avoid using harsh treatments like vortexing or sonicating, as they may denature some of the enzyme.
      3. Use a pipette and sterile wide-bore pipette tip to remove a portion of the suspension.
      4. Transfer the portion to a clean microcentrifuge or centrifuge tube.
      5. Pellet the enzyme by centrifuging at approximately 10,000 – 15,000 x g for 10 minutes, if possible, using a refrigerated microcentrifuge set to a temperature between 2 °C and 8 °C.
      6. Carefully remove as much clear supernatant as possible, retaining it; it is not necessary to remove absolutely all of the ammonium sulfate solution.
      7. Dissolve the pellet by adding an appropriate amount of reaction buffer.
      8. Assay the supernatant and the solution from the pellet for protein content and/or enzyme activity.

      - If the ammonium sulfate will not interfere with the reaction(s), follow the same steps as above.

      It's important to note that for an ammonium sulfate suspension, most of the enzyme will be in solid form, and only negligible amounts of enzyme will likely be in the ammonium sulfate solution.

      Alternatively, there are several Amyloglucosidase enzymes available in solid form (e.g., Product A7420 and A9228) that can be suspended in a solvent of your choice.

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