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5 SETS
₩87,087
50 SETS
₩481,313
₩87,087
출고 가능일2025년 4월 09일세부사항
기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요
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5 SETS
₩87,087
50 SETS
₩481,313
About This Item
₩87,087
출고 가능일2025년 4월 09일세부사항
기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요
추천 제품
제품명
SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL
양식
tablet
solubility
deionized water: soluble 5 mL
저장 온도
−20°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
애플리케이션
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
- to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)[1]
- to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay[2]
- as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)[3]
- as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin[4]
재구성
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.
법적 정보
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Conall H Watson et al.
PLoS neglected tropical diseases, 11(7), e0005786-e0005786 (2017-07-21)
Fiji, an upper-middle income state in the Pacific Ocean, has experienced an increase in confirmed case notifications of enteric fever caused by Salmonella enterica serovar Typhi (S. Typhi). To characterize the epidemiology of typhoid exposure, we conducted a cross-sectional sero-epidemiological
Zimu Wu et al.
International journal of clinical and experimental pathology, 12(5), 1757-1763 (2020-01-15)
Post-stroke induction of alpha-synuclein (AS), a neuronal protein implicated in the pathogenesis of Parkinson's disease (PD), has been demonstrated to induce secondary brain damage after cerebral ischemia. Therefore, understanding the expression and pathogenic modifications of AS is clinically meaningful for
Ruklanthi de Alwis et al.
The Journal of infectious diseases, 219(2), 295-304 (2018-10-16)
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Ronit Rosenfeld et al.
Nature communications, 12(1), 944-944 (2021-02-13)
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), exhibits high levels of mortality and morbidity and has dramatic consequences on human life, sociality and global economy. Neutralizing antibodies constitute a highly promising
Yuankun Zhai et al.
Journal of cellular physiology, 234(10), 18602-18614 (2019-03-27)
Homocysteine (Hcy) is detrimental to bone health in a mouse model of diet-induced hyperhomocysteinemia (HHcy). However, little is known about Hcy-mediated osteoblast dysfunction via mitochondrial oxidative damage. Hydrogen sulfide (H2 S) has potent antioxidant, anti-inflammatory, and antiapoptotic effects. In this
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
프로토콜
간접 ELISA 및 포획 ELISA 실시에 관한 상세한 지침을 제공하는 ELISA 프로토콜 샌드위치 ELISA 및 간접 ELISA 실험을 실시하기 위한 권장 제품과 기법에 대해 자세히 알아봅니다.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
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