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Merck
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문서

21888

Sigma-Aldrich

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

동의어(들):

CFSE

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모든 사진(1)

About This Item

실험식(Hill 표기법):
C29H19NO11
CAS Number:
150347-59-4
Molecular Weight:
557.46
MDL number:
MFCD00037466
UNSPSC 코드:
12352108
PubChem Substance ID:
57647951
NACRES:
NA.32

제품 라인

BioReagent

분석

≥90% (HPLC)

형태

solid

mp

152-154 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

형광

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

적합성

suitable for fluorescence

저장 온도

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

일반 설명

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

애플리케이션

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.
5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
  • to label cells for flow cytometry
  • in lymphocyte proliferation assay
  • to determine the intracellular or intra-organellar pH in chloroplasts
  • to label Leishmania amazonensis and Leishmania major

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

생화학적/생리학적 작용

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

관련 제품

제품 번호
설명
가격

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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