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Merck
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주요 문서

SCT106

Sigma-Aldrich

BioTracker 490 Green Cytoplasmic Membrane Dye

Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.

동의어(들):

Cytoplasmic membrane dye, Live cell imaging probe

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크기 선택

1 ML
₩506,891

₩506,891

구입 가능 여부는 고객센터에 문의하십시오.
모든 사진(1)

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보기 변경
1 ML
₩506,891

About This Item

UNSPSC 코드:
12352207
NACRES:
NA.47

₩506,891

구입 가능 여부는 고객센터에 문의하십시오.

기술

cell based assay: suitable

검출 방법

fluorometric

배송 상태

ambient

일반 설명

Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.

Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.

Spectral Properties
Absorbance: 484nm
Emission: 501nm

애플리케이션

Live cell fluorescent imaging
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

품질

Spectral Properties
Absorbance: 484nm
Emission: 501nm

물리적 형태

Liquid

저장 및 안정성

Store BioTracker 490 Green Cytoplasmic Membrane Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

FlameExclamation mark
GHS02,GHS07

신호어

Danger

유해 및 위험 성명서

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

55.4 °F

Flash Point (°C)

13 °C

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

질문

  1. I am already using the BioTracker NIR750 and I am very satisfied. For this product I don't see the Data Sheet. At which concentration is it delivered? Which is the optimal final concentration to use? For how many round of cell division can be detected?

    1 답변

    1. The product is supplied as a 200 X solution. The specific formulation is considered proprietary. The dye is added at a rate of 5 ul per mL of culture medium. The lipophilic carbocyanine dyes have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability and do not readily transfer between cells with intact membranes which allows for use in cell migration and tracking studies of mixed cell populations. The stability of labeling varies based on cell types and the rate of membrane turnover or cell division. Please see the link below to review a product Certificate of Analysis which serves as the datasheet:
      https://www.emdmillipore.com/US/en/product/BioTracker-490-Green-Cytoplasmic-Membrane-Dye,MM_NF-SCT106#documentation

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