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Merck
모든 사진(1)

주요 문서

62971

Sigma-Aldrich

Lysozyme from chicken egg white

powder (crystalline), 70000-140000 U/mg

동의어(들):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.22
제품 62971은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.

생물학적 소스

chicken egg white

Quality Level

양식

powder (crystalline)

특이 활성도

70000-140000 U/mg

분자량

single-chain 14.3 kDa
Mr ~14600

기술

cell based assay: suitable

적합성

suitable for cell lysis

UniProt 수납 번호

응용 분야

cell analysis

저장 온도

2-8°C

유전자 정보

chicken ... LYZ(396218)

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애플리케이션

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

생화학적/생리학적 작용

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.

단위 정의

1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Steffi Rudolph et al.
Food chemistry, 224, 19-25 (2017-02-06)
Enzymatic hydrolysis of proteins from rice, soy, pea and wheat, with both chymotrypsin and thermolysin, resulted in hydrolysates, which are efficient inhibitors of the angiotensin-converting enzyme (ACE). IC
Guangping Huang et al.
Science China. Life sciences, 64(1), 88-95 (2020-07-04)
Gut microbiota plays a critical role in host physiology and health. The coevolution between the host and its gut microbes facilitates animal adaptation to its specific ecological niche. Multiple factors such as host diet and phylogeny modulate the structure and
Theresa Pesch et al.
Frontiers in immunology, 10, 2630-2630 (2019-12-05)
Immune cell therapies based on the integration of synthetic antigen receptors comprise a powerful strategy for the treatment of diverse diseases, most notably T cells engineered to express chimeric antigen receptors (CAR) for targeted cancer therapy. In addition to T
Rizwan Mahmud et al.
Journal of molecular microbiology and biotechnology, 29(1-6), 43-56 (2019-12-19)
The human gut is inhabited by several hundred different bacterial species. These bacteria are closely associated with our health and well-being. The composition of these diverse commensals is influenced by our dietary intakes. Non-caloric artificial sweeteners (NAS) have gained global
Senlin Shao et al.
Water research, 159, 95-101 (2019-05-13)
Due to the lack of robust ways to quantify aggregations, fouling of two-foulant aggregations is poorly understood. This work systematically reports the ultrafiltration membrane fouling by aggregations formed from two oppositely charged organic foulants (i.e., humic acid (HA) and lysozyme

질문

1–2 / 2 질문  
  1. What is the Assay and Purity of the product?

    1 답변
    1. This product has been discontinued and is no longer available. Other lysozyme products are available and may be tested for purity as well as activity. The information available would be product dependent.

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  2. From which buffer was the product lyophilized?

    1 답변
    1. This product is purified by ion-exchange and dialyzed to remove salt. It is 3X crystallized first in KCl and then in NaCl in its final stage.

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