HybriScan™D kits detect and simultaneously identify microorganisms using rRNA as the detection target. The principle is based on in situ sandwich hybridization with two specific probes. As the rRNA of dead cells is degraded in the presence of RNase, only living cells are detected. Each cell has several thousand rRNA copies, so neither PCR nor a polymerase is needed for this technology, which means that this molecular biology test is not inhibited by the sample matrix, for example food, beverages and water. We recommend a 24-hour enrichment in MRS Broth (Cat. No. 1.10661), Tomato Juice Broth (Cat. No. 17218)