NGLYFL-RO

N-Glycosidase F

recombinant form of the gene from Flavobacterium meningosepticum

• 동의어(들): N-Glycosidase F, PNGase F, Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase
• 효소 위원회 번호: 3.5.1.52 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bacterial (Flavobacterium meningosepticum)
• Quality Level: 100
• 재조합: expressed in E. coli
• 결합: (N-linked)
• 분석: ≥90% (SDS-PAGE)
• 형태: lyophilized
• 특이 활성도: >25000 units/mg protein
• 분자량: 35.5 kDa
• 정제법: electrophoresis
• 포장: pkg of 100 U (11365185001); pkg of 250 U (11365193001)
• 제조업체/상표: Roche
• 저장 조건: (Keep container tightly closed in a dry and well-ventilated place.)
• 기술: activity assay: suitable
• 색상: colorless
• 최적 pH: 7.0-8.0
• solubility: water: soluble
• 적합성: suitable for enzyme test
• 외래 활성: Endoglycosidase F, none detected; a-Fucosidase, present; b-Galactosidase, present; b-N-Acetylhexosaminidase, present; dA(17h ≤100 units, present
• 저장 온도: 2-8°C

설명

일반 설명

N-Glycosidase F (PNGase F) is a potent enzyme which hydrolyzes at glycosylamine linkage. It also helps in generating a carbohydrate-free peptide and oligosaccharide with di-N-acetylchitobiose unit.

N-glycosidase F, also known as PNGase F, is an asparagine amidase enzyme derived from Flavobacterium meningosepticum. It is widely used as a valuable tool in protein research to investigate and analyze N-glycosylation.

특이성

Hydrolyzes all types of N-glycan chains from glycopeptides and glycoproteins unless they carry α1,3-linked core fucose residues present in insect and plant glycoproteins. Free of contaminating proteolytic activities (x = H or sugar[s]) according to current quality control procedures.

애플리케이션

N-Glycosidase F has been used for deglycosylation of N-glycoproteins.

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a solution.

단위 정의

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycoprotein within 1 minute at 37 °C and pH 7.8.

물리적 형태

Clear, colorless solution after reconstitution

제조 메모

Storage conditions (working solution): 2 to 8 °C
The reconstituted solution is stable at 2 to 8 °C for at least four weeks.

재구성

Dissolving the content in 0.1 ml redist water (100 unit package) or 0.25 ml double-dist. water (250 unit package) respectively, results in a concentration of 100 mM sodium phosphate buffer, 25 mM EDTA, pH 7.2.
Note: N-Glycosidase F, recombinant is also available as solution with 50% glycerol.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash