P7367

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE)

• 동의어(들): N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
• CAS Number: 83534-39-8
• 효소 위원회 번호: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD00131222
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 생물학적 소스: bacterial (Elizabethkingia meningoseptica)
• Quality Level: 200
• 결합: (N-linked)
• 제품 라인: BioReagent
• 분석: ≥95% (SDS-PAGE)
• 양식: powder
• 유통기한: ≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml); ≥1 yr at 2‑8 °C; Solution is stable for at least 3 freeze-thaw cycles
• 분자량: ~36 kDa
• 농도: ≥300 units/mL; ≥50 units/mL
• 최적 pH: ~8.6
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

애플리케이션

PNGase F from Elizabethkingia meningoseptica has been used:

• for de-N-glycosylation of Zika20virus E protein[1]
• to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells[2]
• to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)[3]

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis

Used to deglycosylate protein.

생화학적/생리학적 작용

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

단위 정의

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)