₩511,865
출고 가능일2025년 4월 28일세부사항
추천 제품
생물학적 소스
bacterial (Arthrobacter ureafaciens)
Quality Level
양식
solution
특이 활성도
~25 units/mg protein
포장
pkg of 1 U (100 μl)
제조업체/상표
Roche
최적 pH
5.0-5.5
저장 온도
2-8°C
일반 설명
Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.[1]
특이성
Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.
애플리케이션
For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
물리적 특성
This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.
물리적 형태
Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7
제조 메모
Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.
기타 정보
For life science research only. Not for use in diagnostic procedures.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
WGK
nwg
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
이미 열람한 고객
Tomonori Kaifu et al.
The Journal of experimental medicine, 218(12) (2021-11-25)
Dendritic cell immunoreceptor (DCIR) is a C-type lectin receptor with a carbohydrate recognition domain and an immunoreceptor tyrosine-based inhibitory motif. Previously, we showed that Dcir-/- mice spontaneously develop autoimmune enthesitis and sialadenitis, and also develop metabolic bone abnormalities. However, the
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The liver has recently been identified as a major organ for destruction of desialylated platelets. However, the underlying mechanism remains unclear. Kupffer cells, which are professional phagocytic cells in the liver, comprise the largest population of resident tissue macrophages in
Jiaqi Wu et al.
BioTechniques, 68(2), 85-90 (2020-01-16)
Carbohydrate-deficient transferrin (CDT) is a reliable biomarker for chronic alcohol abuse. We developed a method for CDT analysis by capillary isoelectric focusing, followed by immunodetection directly in the capillary, in an automated fashion and on a single platform (Peggy Sue™;
Sialylation by ??galactoside ??2,6?sialyltransferase and N?glycans regulate cell adhesion and invasion in human anaplastic large cell lymphoma
Suzuki O, et al.
International Journal of Oncology, 46(3), 973-980 (2015)
프로토콜
Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.
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