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Merck
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11815016001

Roche

Anti-HA Affinity Matrix

from rat IgG1

동의어(들):

affinity matrix, anti-ha

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About This Item

UNSPSC 코드:
12352203
가격 및 재고 정보를 현재 이용할 수 없음 고객지원팀으로 연락바랍니다.

생물학적 소스

rat

Quality Level

100

결합

agarose conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

3F10, monoclonal

양식

slurry

포장

pkg of 1 mL (settled resin volume)

제조업체/상표

Roche

동형

IgG1

에피토프 서열

YPYDVPDYA

용량

2-8 nmol/mL binding capacity

저장 온도

2-8°C

관련 카테고리

일반 설명

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)

특이성

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).

면역원

Amino acids 98-106 from the human influenza virus hemagglutinin protein

애플리케이션

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
  • Western blotting using the Anti-HA antibody
  • Silver staining (or similar protein stain)
  • In coimmunoprecipitation.[1]
Use Anti-HA Affinity Matrix for:
  • Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts[2][3]
  • Affinity column purification of HA-tagged proteins from crude protein extracts

특징 및 장점

  • Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
  • Use nondenaturing conditions for elution of your HA-tagged protein of choice.
  • Purify even rarely expressed HA-tagged proteins.
  • Highly specific to YPYDVPDYA.
  • Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
  • Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.

품질

Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.

물리적 형태

1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.

분석 메모

Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.

기타 정보

For life science research only. Not for use in diagnostic procedures.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
55087000
41826600
35964200
73946700
64553700

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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Anti-HA Affinity Matrix Protocol & Troubleshooting

Anti-HA Affinity Matrix Protocol & Troubleshooting

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