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추천 제품
사용
sufficient for 96 reactions
제조업체/상표
Roche
배송 상태
dry ice
저장 온도
−20°C
특이성
The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.
애플리케이션
특징 및 장점
Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
- High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
- Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
- Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
- Fast results: The entire procedure can be performed in one day
- Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing
포장
1 kit containing 14 components.
서열
Chain Length Positive control: 520 bp.
기타 정보
For life science research only. Not for use in diagnostic procedures.
키트 구성품 전용
제품 번호
설명
- Lysis Reagent
- Neutralization Reagent
- PCR Mix ready-to-use
- Denaturation Reagent
- Hybridization Buffer
- Biotinylated Capture Probe
- Washing Buffer 10x concentrated
- Anti-Digoxigenin-POD antibody
- Conjugate Dilution Buffer
- TMB Substrate Solution
- Stop Reagent
- Positive Control DNA
- Streptavidin-coated Microplate
- Adhesive Plate Cover Foils
모두 보기 (14)
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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프로토콜
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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