콘텐츠로 건너뛰기
Merck
모든 사진(1)

문서

MP0035

Sigma-Aldrich

LookOut® Mycoplasma PCR Detection Kit

Optimized for use with JumpStart Taq DNA Polymerase, D9307.

동의어(들):

PCR-based mycoplasma screening

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

품질

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

포장

pkg of 1 kit

기술

PCR: suitable

응용 분야

detection
microbiology

호환성

Optimized for use with JumpStart Taq DNA Polymerase, D9307.

저장 온도

2-8°C

일반 설명

The LookOut® Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals. Kit has been successfully tested for the ability to amplify Mycoplasma fermentans genomic DNA in concentrations of 20,000 to 20 copies.
The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye. Total assay time is greatly reduced compared to general protocols that require individual loading of reaction tubes.

애플리케이션

The LookOut® Mycoplasma PCR Detection Kit has been used for screening of mycoplasma infection.

호환성

The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3′-5′ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

법적 정보

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

관련 제품

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Dmitri Pchejetski et al.
Cancer research, 70(21), 8651-8661 (2010-10-21)
Radiotherapy is widely used as a radical treatment for prostate cancer, but curative treatments are elusive for poorly differentiated tumors where survival is just 15% at 15 years. Dose escalation improves local response rates but is limited by tolerance in
Nellie Moshkovich et al.
Cancer research, 80(11), 2125-2137 (2020-04-09)
Peptidylarginine deiminases (PADI) catalyze posttranslational modification of many target proteins and have been suggested to play a role in carcinogenesis. Citrullination of histones by PADI4 was recently implicated in regulating embryonic stem and hematopoietic progenitor cells. Here, we investigated a
Soudabeh Rad Pour et al.
Frontiers in oncology, 10, 51-51 (2020-03-03)
Dysregulation of the kynurenine pathway has been regarded as a mechanism of tumor immune escape by the enzymatic activity of indoleamine 2, 3 dioxygenase and kynurenine production. However, the immune-modulatory properties of other kynurenine metabolites such as kynurenic acid, 3-hydroxykynurenine
Lucian Romeo Farcal et al.
Nanotoxicology, 7(6), 1095-1110 (2012-07-10)
An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of
Jennifer Mary Wymant et al.
Journal of Cancer, 11(11), 3288-3302 (2020-04-02)
Efficacious anticancer therapies for targeting plasma membrane receptors with antibody based therapeutics are often contingent on sufficient endocytic delivery of receptor and conjugate to lysosomes. This results in downregulation of receptor activity and, in the case of antibody-drug conjugates (ADCs)

문서

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Overview of good cell banking practices for cell line cryopreservation purposes.

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

모두 보기

프로토콜

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

관련 콘텐츠

머크의 포괄적인 우태혈청(FBS) 포트폴리오에서 귀하의 실험실 요건과 예산에 적합한 제품을 찾아보십시오. 지구촌의 문제가 FBS 가격에 미치는 영향에 대해 알아보십시오.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.