11333054001

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

>90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

• 동의어(들): Digoxigenin NHS-ester, dig-nhs-ester, ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester, DIG NHS
• 실험식(Hill 표기법): C₃₅H₅₀N₂O₁₀
• CAS Number: 129273-26-3
• Molecular Weight: 658.78
• MDL number: MFCD02093394
• UNSPSC 코드: 41105600
• PubChem Substance ID: 329749387

속성

• 분석: >90% (elementary analysis)
• 형태: powder
• 분자량: M_r 658.8
• 포장: pkg of 5 mg
• 제조업체/상표: Roche
• pH 범위: 2-13
• solubility: DMF: soluble; DMSO: soluble; ethanol: soluble
• 저장 온도: −20°C
• SMILES string: [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
• InChI: 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
• InChI key: KHNDABJZSPPYLE-FUGFVFQCSA-N

설명

일반 설명

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

애플리케이션

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).

생화학적/생리학적 작용

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

원리

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

제조 메모

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS06,GHS08,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H300,H319,H331,H373,H410
• 예방조치 성명서: P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
• Hazard Classifications: Acute Tox. 2 Oral - Acute Tox. 3 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - STOT RE 2
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable