추천 제품
생물학적 소스
sheep
Quality Level
결합
peroxidase conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
양식
lyophilized
포장
pkg of 50 U
제조업체/상표
Roche
동형
IgG
저장 온도
2-8°C
일반 설명
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.[1] Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.[2] This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.
특이성
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
애플리케이션
Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
- whole-mount in situ hybridization[3]
- fluorescence in situ hybridization[4]
- enzyme linked immunosorbent assay (ELISA)[5]
- dot blot
- Southern blot
- western blot
- double labeling experiments
- lectin blots
Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
제조 메모
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
- Dot blot: 50 to 100 mU/ml
- ELISA: 2 to 50 mU/ml
- Western blot: 50 to 100 mU/ml
- Southern blot: 50 to 100 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
재구성
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
기타 정보
For life science research only. Not for use in diagnostic procedures.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
이미 열람한 고객
In Situ Hybridization Methods (2015)
Christopher R Campos et al.
PloS one, 15(3), e0229850-e0229850 (2020-03-11)
Reducing Amyloid β (Aβ) in the brain is of fundamental importance for advancing the therapeutics for Alzheimer`s disease. The endogenous metallopeptidase neprilysin (NEP) has been identified as one of the key Aβ-degrading enzymes. Delivery of NEP to the brain by
Potent and selective BACE-1 peptide inhibitors lower brain Abeta levels mediated by brain shuttle transport
<BIG>Ruderisch N, et al.</BIG>
EBioMedicine, 24, 76-92 (2017)
Monica Musiani et al.
Nature protocols, 2(10), 2511-2519 (2007-10-20)
Competitive PCR-ELISA combines competitive PCR with an ELISA to allow quantitative detection of PCR products. It is based on the inclusion of an internal standard competitor molecule that is designed to differ from the target by a short sequence of
Louise Heydenreich Jensen et al.
International archives of allergy and immunology, 178(4), 307-314 (2019-02-14)
Infant formulas (IFs) based on hydrolysed cow's milk proteins are central in the management of cow's milk allergy (CMA) in infants and small children. New IF compositions with improved prevention and treatment properties are needed, along with appropriate preclinical animal
문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
