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Merck
모든 사진(8)

문서

T3824

Sigma-Aldrich

Tyrosinase from mushroom

lyophilized powder, ≥1000 unit/mg solid

동의어(들):

Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase

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About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

fungus (mushroom)

형태

lyophilized powder

특이 활성도

≥1000 unit/mg solid

분자량

119.5 kDa by electrophoresis
128 kDa by sedimentation velocity diffusion
133 kDa by LS

응용 분야

diagnostic assay manufacturing

배송 상태

wet ice

저장 온도

−20°C

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일반 설명

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper.

애플리케이션

Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population.

생화학적/생리학적 작용

Converts tyrosine to L-DOPA or tyramine to dopamine

물리적 특성

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

단위 정의

One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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A T S Jorge et al.
International journal of cosmetic science, 34(5), 435-440 (2012-06-29)
Several treatments for skin whitening are available today, but few of them are completely adequate, especially owing to the carcinogenic potential attributed to classical drugs like hydroquinone, arbutin and kojic acid. To provide an alternative and safer technology for whitening
Shilpi Goenka et al.
Molecules (Basel, Switzerland), 25(16) (2020-08-13)
Previous studies have reported that estrogen hormone promotes melanogenesis while progesterone inhibits it. A selective estrogen receptor modulator (SERM), tamoxifen, has been shown to promote melanogenesis; however, to date, there have been no reports on the effects of a selective
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Zhang, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 22, 83-90 (2019)
Thomas J Jaworek et al.
Orphanet journal of rare diseases, 7, 44-44 (2012-06-28)
Oculocutaneous albinism (OCA) is caused by a group of genetically heterogeneous inherited defects that result in the loss of pigmentation in the eyes, skin and hair. Mutations in the TYR, OCA2, TYRP1 and SLC45A2 genes have been shown to cause
Rongchao Zhang et al.
Evidence-based complementary and alternative medicine : eCAM, 2020, 2582579-2582579 (2020-08-11)
Whitening cosmetics market has a bright future, and pure natural whitening products of traditional Chinese medicine have always been a research hotspot. In this research, the whitening active ingredient of Chinese medicine Trichosanthes pulp was isolated and purified for the

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