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Merck
모든 사진(2)

문서

SCM301

Sigma-Aldrich

3dGRO® Organoid Freeze Medium

Optimized cryopreservation media for multiple organoids cell types.

동의어(들):

Organoid cryopreservation medium

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About This Item

UNSPSC 코드:
12352207
eCl@ss:
32160801
NACRES:
NA.71

형태

liquid

기술

cell culture | stem cell: suitable

일반 설명

Organoids are in-vitro derived 3D cell aggregates derived from primary tissue or stem cells that are capable of self-renewal, self-organization and exhibit organ functionality. Organoids address the limitations of existing 2D model systems by providing:

Similar composition and architecture to primary tissue: Organoids harbor small population of self-renewing stem cells (such as intestinal crypt stem cells) that can differentiate into cells of all major cell lineages, with similar frequency as in physiological conditions.
Relevant models of in-vivo conditions: Organoids are more biologically relevant to any model system and are amenable to manipulate niche components and gene expression.
Stable system for extended cultivation: Organoids can be cryopreserved as biobanks and expanded indefinitely by leveraging self-renewal and the differentiation capability of the stem cell populations.

Organoids can be cryopreserved using standard cell freezing techniques however freeze/thaw viability remains a challenge. The 3dGRO Organoid Freeze Medium is a proprietary optimized cryopreservation media for multiple organoids cell types (etc. mouse intestinal organoids, human colon and lung organoids). The media supports higher freeze/thaw viabilities compared to other commercial or homemade organoid freezing media formulations.

애플리케이션

Optimized cryopreservation media for multiple organoids cell types.
Research Category
Cell Culture

Stem Cell Research

품질

Appearance (color): Amber
Osmolality: 250-350 mOsm
pH: 6.8-7.1
Sterility Tested: No Growth/Pass
Bacterial & Fungal Tested: Passed

저장 및 안정성

Store the 3dGRO Organoid Freeze Medium at -20°C. Aliquot into smaller working aliquots before use to avoid reagent contamination issues.

법적 정보

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

조직 및 줄기세포 유래 3D 뇌, 장, 소화관, 폐 및 암 종양 오가노이드 모델을 생성하는 오가노이드 배양 제품.

모두 보기

프로토콜

How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies

어떻게 오가노이드를 염색합니까? 항체를 이용한 오가노이드 배양에서 면역형광(IF) 및 면역세포화학(ICC) 염색에 대한 완전한 단계별 프로토콜

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.