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Merck
모든 사진(1)

문서

SCM162

Sigma-Aldrich

DMEM/F-12 PLUS Basal Medium

Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.

동의어(들):

Advanced DMEM/F12, Organoid Basal Media, Organoid Basal Media, DMEMF12, DMEM F12, Dulbecco′s Modified Eagle Medium/Ham′s F-12, Serum-Free Basal Media

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About This Item

UNSPSC 코드:
12352207
eCl@ss:
32160801
NACRES:
NA.71

형태

liquid

기술

cell culture | stem cell: suitable

일반 설명

DMEM/F-12 PLUS is an advanced formulation of Dulbecco′s Modified Eagle Medium/Ham′s F-12 basal media that can be used in a broad array of cell lines for applications that require reduced or no serum supplementation. Cell lines cultured in DMEM/F12 PLUS and reduced serum exhibit equivalent or superior cell growth while maintaining the same cell morphology and function as in high serum conditions. Applications for DMEM/F12 PLUS include but are not limited to the following:

• Culture of primary, stem cells and cell lines in applications that require reduced or serum-free medium.
• Optimization of transfections that require reduced or serum-free conditions.
• Production of conditioned medium in reduced serum or serum-free conditions.
• Optimized media for the culture and expansion of multiple 3D organoids and tissues systems.

DMEM/F12 PLUS is a proprietary formulation that contains human serum albumin (HSA), defined lipids, trace elements, chemicals and proteins essential for optimal cell growth in reduced serum conditions. The media contains high-glucose and does not contain L-glutamine or penicillin-streptomycin.

애플리케이션

Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.
Research Category
Stem Cell Research

품질

Appearance (color): Clear, Red Liquid
Osmolality: 290-330 mOsm
pH: 7.2-7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative

저장 및 안정성

Store at 2-8°C. Before use, aliquot into smaller volumes to avoid contamination. Protect from light when not in use. Acclimate the medium at room temperature for 15-20 minutes before use. Do not warm media at 37°C before use.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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