A stock solution was prepared at 5 mM in DMSO as a 5000X concentrated solution from cat# T5648 before adding it to the cell culture medium. The cells are thawed and expanded in the MCF-7/TAMR-1 Expansion Media, which includes DMEM/F12 medium without phenol red (Sigma Cat. No. D6434), 1% FBS (EMD Millipore Cat. No. ES-009-B), 2.5 mM L-Glutamine (EMD Millipore Cat. No. TMS-002-C), 6 ng/mL insulin (Sigma Cat. No. I-9278), and 1 uM tamoxifen (Sigma Cat. No. T5648).
SCC101
MCF-7/TAMR-1 Human Breast Cancer Cell Line
Human
동의어(들):
MCF7 TAMR-1
Human breast cancer cell line
Tamoxifen resistant breast cancer cell line, MCF7 TAMR-1 Human breast cancer cell line Tamoxifen resistant breast cancer cell line
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모든 사진(1)
About This Item
UNSPSC 코드:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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추천 제품
제품명
MCF-7/TAMR-1 Human Breast Cancer Cell Line, MCF-7/TAMR-1 is a tamoxifen-resistant cell line derived from the MCF-7/S0.5 human breast cancer cell line by long term treatment with the drug tamoxifen.
생물학적 소스
human
Quality Level
기술
cell culture | mammalian: suitable
배송 상태
ambient
일반 설명
Estrogen receptor-positive breast cancer is the most common type of breast cancer. About 80% of all breast cancers express the estrogen receptor (ER+) and depend on estrogen to support the growth and spread of the cancer cells. Hormonal therapy, such as tamoxifen, may help slow or stop the growth of ER+ breast cancers by blocking the actions of estrogen. Primary or acquired tamoxifen resistance poses a major clinical challenge in the treatment of ER+ breast cancer. The MCF-7/TAMR-1 cell line provides a model cell system for studying tamoxifen resistance, elucidating signaling pathways involved in tamoxifen-resistant growth, and identifying new predictive markers for response to hormonal therapy.
MCF-7/TAMR-1 is a tamoxifen-resistant cell line derived from the MCF-7/SO.5 cell line that has been adapted to grow at low serum concentration under the long-term treatment with 1 M with tamoxifen. MCF-7/TAMR-1 cells should be maintained in 1 M tamoxifen and may be compared with the parental MCF-7/S0.5 cells, which are sensitive to tamoxifen.
MCF-7/TAMR-1 is a tamoxifen-resistant cell line derived from the MCF-7/SO.5 cell line that has been adapted to grow at low serum concentration under the long-term treatment with 1 M with tamoxifen. MCF-7/TAMR-1 cells should be maintained in 1 M tamoxifen and may be compared with the parental MCF-7/S0.5 cells, which are sensitive to tamoxifen.
세포주 설명
Cancer Cells
애플리케이션
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact [email protected].
품질
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
저장 및 안정성
MCF-7/TAMR-1 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
188.6 °F - closed cup - (Dimethylsulfoxide)
Flash Point (°C)
87 °C - closed cup - (Dimethylsulfoxide)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
P Briand et al.
Cancer research, 44(3), 1114-1119 (1984-03-01)
The human breast cancer cell line MCF-7 has been adapted to long-term growth at 0.5 and 0.05% fetal bovine serum. Free cytoplasmic and filled nuclear estrogen receptors were found in cells grown at 5, 0.5, and 0.05% fetal bovine serum.
A E Lykkesfeldt et al.
British journal of cancer, 53(1), 29-35 (1986-01-01)
The human breast cancer cell line MCF-7 requires oestrogen to produce and promote growth of tumours in athymic mice. In vitro, however, MCF-7 cells proliferate rapidly without supply of oestrogen (Briand & Lykkesfeldt, 1984). Oestrogen stimulation of proliferation of MCF-7
M W Madsen et al.
Molecular and cellular endocrinology, 109(2), 197-207 (1995-04-01)
Breast cancer patients with an estrogen receptor (ER) positive tumor can be treated with the anti-estrogen tamoxifen, but development of anti-estrogen resistance is a serious problem. We have analyzed a tamoxifen resistant human breast cancer cell line MCF-7/TAMR-1 for alterations
Melissa A Maczis et al.
Journal of lipid research, 59(12), 2297-2307 (2018-10-14)
In breast cancer, 17β-estradiol (E2) plays critical roles mainly by binding to its canonical receptor, estrogen receptor (ER) α66, and eliciting genomic effects. E2 also triggers rapid, nongenomic responses. E2 activates sphingosine kinase 1 (SphK1), increasing sphingosine-1-phosphate (S1P) that binds
Yan Zhao et al.
Aging, 13(14), 18498-18514 (2021-07-23)
Breast cancer (BC) is a common malignant tumor in females. The challenge in treating BC is overcoming chemoresistance. Exosome-mediated transfer of miRNAs is a molecule-shuttle in intercellular communication. Thus, we aimed to investigate whether exosomal miRNA-205 could affect chemoresistance and
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