MAB3806-C
Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free
clone 10H11.E12, from mouse
동의어(들):
Serine-protein kinase ATM, Ser1981 phosphorylated, Ataxia telangiectasia mutated, Ser1981 phosphorylated, A-T mutated, Ser1981 phosphorylated
로그인조직 및 계약 가격 보기
모든 사진(1)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
10H11.E12, monoclonal
종 반응성
mouse, human
기술
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
phosphorylation (pSer1981)
유전자 정보
human ... ATM(472)
일반 설명
Serine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.
특이성
Reacts with ATM Kinase phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
면역원
Epitope: pSer1981.
Synthetic peptide corresponding to a.a. 1974-1988 of human ATM with phosphorylated Ser1981 (SLAFEEG[pS]QSTTISS).
애플리케이션
Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free is an antibody against phospho-ATM for use in Immunocytochemistry, Immunoprecipitation, ELISA, Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Ser1981-phosphorylated ATM interaction with endogenous EphA5 by co-immunoprecipitation using chromatin-enriched protein fractions of irradiated NCI-H460 human lung cancer cells (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
ELISA Analysis: a representative lot was employed as the capture antibody for the detection of interaction between EphA5 and Ser1981-phosphorylated ATM. ATM pSer1981 captured from NCI-H460 human lung cancer cell extracts interacted with recombinant EphA5 cytoplasmic domain, but not with EphA5 extracellular domain or kinase domain (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced upregulation of nuclear ATM pSer1981 foci by fluorescent immunocytochemistry staining of methanol-fixed NCI-H460 human lung cancer cells (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of 1.2% formaldehyde-fixed, 0.1% Triton X-100-permeabilized HT29 cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
Immunocytochemistry Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of methanol/acetone-fixed primary human foreskin fibroblasts (HFFs) (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
Western Blotting Analysis: A representative lot detected epirubicin-induced ATM Ser1981 (Ser1987 in mouse) phosphorylation in mouse embryonic fibroblasts (MEFs) and human MCF-7 cells (Khongkow, P., et al. (2014). Oncogene. 33(32): 4144-4155).
Western Blotting Analysis: A representative lot detected camptothethin-induced ATM Ser1987 (Ser1981 in human) phosphorylation in primary mouse cortical neurons (Brochier, C., et al. (2013). J. Neurosci. 33(20): 8621-8632).
Western Blotting Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation in HT29, HeLa, and HEK293T cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in U2OS cells by Western blotting using whole cell lyates or B56γ immunoprecipitate (Shouse, G.P., et al. (2011). Oncogene. 30(35):3755-3765).
Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in cultured primary human foreskin fibroblasts (HFFs), as well as passage-dependent basal ATM Ser1981 phosphorylation levels in HFFs (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
ELISA Analysis: a representative lot was employed as the capture antibody for the detection of interaction between EphA5 and Ser1981-phosphorylated ATM. ATM pSer1981 captured from NCI-H460 human lung cancer cell extracts interacted with recombinant EphA5 cytoplasmic domain, but not with EphA5 extracellular domain or kinase domain (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced upregulation of nuclear ATM pSer1981 foci by fluorescent immunocytochemistry staining of methanol-fixed NCI-H460 human lung cancer cells (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of 1.2% formaldehyde-fixed, 0.1% Triton X-100-permeabilized HT29 cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
Immunocytochemistry Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of methanol/acetone-fixed primary human foreskin fibroblasts (HFFs) (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
Western Blotting Analysis: A representative lot detected epirubicin-induced ATM Ser1981 (Ser1987 in mouse) phosphorylation in mouse embryonic fibroblasts (MEFs) and human MCF-7 cells (Khongkow, P., et al. (2014). Oncogene. 33(32): 4144-4155).
Western Blotting Analysis: A representative lot detected camptothethin-induced ATM Ser1987 (Ser1981 in human) phosphorylation in primary mouse cortical neurons (Brochier, C., et al. (2013). J. Neurosci. 33(20): 8621-8632).
Western Blotting Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation in HT29, HeLa, and HEK293T cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in U2OS cells by Western blotting using whole cell lyates or B56γ immunoprecipitate (Shouse, G.P., et al. (2011). Oncogene. 30(35):3755-3765).
Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in cultured primary human foreskin fibroblasts (HFFs), as well as passage-dependent basal ATM Ser1981 phosphorylation levels in HFFs (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
품질
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected Camptothecin (Cat. No. 208925) treatment-induced increase of nuclear ATM pSer1981 foci in HeLa cells.
Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected Camptothecin (Cat. No. 208925) treatment-induced increase of nuclear ATM pSer1981 foci in HeLa cells.
표적 설명
350.7 kDa calculated.
물리적 형태
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Alena Svobodová Kovaříková et al.
Epigenetics & chromatin, 16(1), 26-26 (2023-06-16)
RNA modifications have been known for many years, but their function has not been fully elucidated yet. For instance, the regulatory role of acetylation on N4-cytidine (ac4C) in RNA can be explored not only in terms of RNA stability and
Emily Leung et al.
Frontiers in neuroscience, 17, 1225226-1225226 (2023-08-28)
Emerging evidence suggests cellular senescence, as a consequence of excess DNA damage and deficient repair, to be a driver of brain dysfunction following repeated mild traumatic brain injury (rmTBI). This study aimed to further investigate the role of deficient DNA
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