추천 제품
생물학적 소스
mouse
Quality Level
결합
PE
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
4G10, monoclonal
종 반응성
vertebrates, human
제조업체/상표
Milli-Mark®
기술
flow cytometry: suitable
동형
IgG2bκ
배송 상태
wet ice
타겟 번역 후 변형
phosphorylation (pTyr)
유전자 정보
human ... PID1(55022)
일반 설명
Some of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
특이성
Antibody recognizes tyrosine-phosphorylated proteins from all species
Expected to detect tyrosyne phosphorylated proteins in all species.
애플리케이션
Milli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.
품질
Evaluated by flow cytometry using EGF treated versus untreated A431 cells
표적 설명
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
물리적 형태
Purified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
분석 메모
Control
Treated versus untreated A431 cells
Treated versus untreated A431 cells
법적 정보
4G10 is a registered trademark of Upstate Group, Inc.
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Neel H Shah et al.
eLife, 7 (2018-03-17)
The specificity of tyrosine kinases is attributed predominantly to localization effects dictated by non-catalytic domains. We developed a method to profile the specificities of tyrosine kinases by combining bacterial surface-display of peptide libraries with next-generation sequencing. Using this, we showed
Veronika Horkova et al.
Cell reports, 30(5), 1504-1514 (2020-02-06)
Overtly self-reactive T cells are removed during thymic selection. However, it has been recently established that T cell self-reactivity promotes protective immune responses. Apparently, the level of self-reactivity of mature T cells must be tightly balanced. Our mathematical model and experimental data
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