AP200C

Goat Anti-Mouse light chain Antibody, Cy3 conjugate

Chemicon^®, from goat

• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

속성

• 생물학적 소스: goat
• Quality Level: 100
• 결합: CY3 conjugate
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: secondary antibodies
• 클론: polyclonal
• 종 반응성: mouse
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; western blot: suitable
• 동형: IgG
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

특이성

Minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep immunoglobulins.

면역원

Epitope: Kappa light chain

Prepared from purified mouse IgG light chain.

애플리케이션

Suggested dilutions:
Western Blotting: 1:100 -1:800 of the 1 mg/mL stock
ELISA: 1:100 -1:800 of the 1 mg/mL stock

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

This Goat anti-Mouse light chain Antibody, Cy3 conjugate is validated for use in ELISA, WB for the detection of Mouse light chain.

표적 설명

25 kDa

물리적 형태

ImmunoAffinity Purified

Lyophilized powder from a solution with 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer. Purified by immunoaffinity chromatography.

저장 및 안정성

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 유해 및 위험 성명서: H412
• 예방조치 성명서: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3