ABC253

Anti-Beclin-2

from rabbit

• 동의어(들): Beclin2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse
• 포장: antibody small pack of 25 μg
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_001277621
• UniProt 수납 번호: P0DM65
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Becn2(226720)

설명

일반 설명

Beclin-2 (UniProt: P0DM65) is encoded by Becn2 gene (Gene ID: 226720) in murine species. Beclin-2 is a member of the Atg6/Beclin family and is involved in 2 distinct lysosomal degradation pathways and acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. It regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1. Beclin-2 family members are specific to mammals whereas Becln-1 subfamily members are present in more primitive vertebrates. Beclin-2 interacts with ATG14, AMBRA1, UVRAG and PIK3C3/VPS34 and this interaction is not disrupted by starvation. It is expressed in brain, skeletal muscle, placenta, thymus and uterus and expression is lower in liver, testis, stomach, and 17-day-old embryos. Disruption of Beclin-2 is shown to reduce embryonic viability and heterozygous knockout mice show defects in autophagy, increased levels of Cnr1 receptor, elevated food intake, and obesity and insulin resistance. (Ref.: He, C. et al. (2013). Cell 154, 1085-1099).

특이성

This rabbit polyclonal antibody detects Becline-2 in murine cells. It targets an epitope within 14 amino acids from the N-terminal.

면역원

Epitope: unknown

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Beclin-2.

애플리케이션

Anti-Beclin-2 Antibody, Cat. No. ABC253, is a rabbit polyclonal antibdoy that detects Beclin-2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Beclin-2 in human brain (thalamic region) and mouse stomach tissues.

Research Category
Apoptosis & Cancer

품질

Evaluated by Western Blotting in Beclin2 +/+ Mouse Brain (Wild-Type).

Western Blotting Analysis: 2 µg/mL of this antibody detected Beclin-2 in lysate from Beclin-2 +/+ Mouse Brain (Wild-type).

표적 설명

~50 kDa observed; 50.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable