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Merck
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69041

Sigma-Aldrich

B834(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

동의어(들):

Competent cells

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About This Item

UNSPSC 코드:
41106202
NACRES:
NA.81
가격 및 재고 정보를 현재 이용할 수 없음 고객지원팀으로 연락바랍니다.

제품명

B834(DE3) Competent Cells - Novagen, B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

생물학적 소스

Escherichia coli

제조업체/상표

Novagen®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

성장 모드

adherent or suspension

형태학

rod shaped

기술

microbiological culture: suitable

세포 형질전환

transformation efficiency: >2×106 cfu/μg

배송 상태

dry ice

저장 온도

−70°C

일반 설명

B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F-ompT hsdSB(rB- mB-) gal dcm met (DE3)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

성분

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlB834(DE3)Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

경고

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

기타 정보

1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

시험 성적서(COA)

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질문

  1. Is this strain protease-free? If no what all proteases are present in it?

    1 답변

    1. This product is derived from a B strain deficient in Lon and OmpT proteases. B834, a protease-deficient host is a methionine auxotroph and allows high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

      Please see the link below to access the User Guide for this product:
      https://www.emdmillipore.com/US/en/product/B834DE3-Competent-Cells-Novagen,EMD_BIO-69041#anchor_USP

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