605190-M

Thrombin, Human Plasma

• 동의어(들): Thrombin, Human Plasma
• CAS Number: 9002-04-4
• MDL number: MFCD00007020
• UNSPSC 코드: 12352202
• NACRES: NA.51

속성

• 생물학적 소스: human plasma
• Quality Level: 100
• 양식: lyophilized
• 특이 활성도: ≥1000 NIH units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• solubility: water: 1 mg/mL; aqueous buffer: soluble
• 저장 온도: −20°C

설명

일반 설명

Thrombin, a sodium-activated type II enzyme, comprises two anion binding exosites, ABE-I and ABE-II.[1] This serine protease enzyme is synthesized from zymogen prothrombin (factor II) in the liver.[1][2]

애플리케이션

Thrombin, Human Plasma has been used:

• as a component of endothelial growth medium (EGM) media for the transplantation and reisolation of Kaposi′s sarcoma-associated herpesvirus-human endothelial cell line (KSHV-HuARLT) cells from mice[3]
• for the fabrication of fibrin gels[4]
• as a component of EGM media for viral copy number analysis of KSHV-HuARLT cells and matrigel implant[5]

생화학적/생리학적 작용

Thrombin cleaves and converts fibrinogen into fibrin. It then activates factors V, VIII, XI, and XIII. Thrombin stimulates platelet activation and stabilizes the fibrin polymers.[6][7] It elicits a vital role in the last stages of the blood coagulation cascade.[1]

경고

Toxicity: Harmful (C)

단위 정의

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

물리적 형태

Lyophilized from 200 mM NaCl, 50 mM citrate buffer, 0.1% PEG-8000, pH 6.5. Contains BSA as a stabilizer.

제조 메모

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

재구성

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 2 months at -70°C.

분석 메모

Complete activation from homogeneous prothrombin by SDS-PAGE

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable