218725

Caspase-5, Human, Recombinant, E. coli

Caspase-5, Human, Recombinant, is expressed in E. coli. It is an inflammatory caspase.

• 동의어(들): ICErel-III, TY
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 분석: ≥80% (SDS-PAGE)
• 양식: liquid
• 특이 활성도: ≥100,000 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

M.W. 10,000 and 22,000 (heterodimer).

Recombinant, human caspase-5 expressed in E. coli. A member of the caspase-1 subfamily of cysteine proteases involved in Fas-mediated apoptosis. Caspase-5 cleavage site is reported to be Asp³¹¹ and Asp³³⁰. May also cleave at Asp¹²¹. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4.

물리적 형태

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

재구성

After initial defrost, aliquot and freeze at -70°C.

기타 정보

Schwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2