C1224

Caspase 3 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

• 동의어(들): Apopain, CPP32, Yama
• 효소 위원회 번호: 3.4.22.56 (BRENDA, IUBMB)
• MDL number: MFCD01634593
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 재조합: expressed in E. coli (C-terminal histidine-tagged)
• Quality Level: 200
• 분석: ≥90% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 특이 활성도: ≥1.0 units/mg protein
• 분자량: ~30 kDa
• UniProt 수납 번호: P42574
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CASP3(836)

설명

일반 설명

Research area: Apoptosis

Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.

애플리케이션

Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay.

This product may also be used in cell-based apoptosis assay.

생화학적/생리학적 작용

Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.

단위 정의

One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.

물리적 형태

Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS

제조 메모

Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable