14-601-M

ROKβ/ROCK-I Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535, autoactivated by incubating with Mg/ATP & redialysed to remove excess ATP. For use in Kinase Assays.

• 동의어(들): Rho-associated protein kinase, p160ROCK
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in baculovirus infected Sf21 cells
• 양식: liquid
• 유통기한: 6 mo.
• 분자량: M_w 61.4 kDa
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• NCBI 수납 번호: NM_005406.2
• UniProt 수납 번호: Q13464
• 저장 온도: −70°C
• 유전자 정보: human ... ROCK1(6093)

설명

일반 설명

Research area: Apoptosis

N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535; autoactivated by incubating with Mg/ATP and redialysed to remove excess ATP

Product Source: expressed by baculovirus in Sf21 insect cells

애플리케이션

Research Category
Inflammation & Immunology

생화학적/생리학적 작용

G protein, RhoA/Rho kinase 1 (ROCK1) [1] is a serine/threonine kinase. It plays a vital role in the regulation of cell growth, and invasive and metastatic progression of various types of human cancers. ROCK1 is involved in the regulation of apoptosis or programmed cell death in various cell types.[2] Overexpression of ROCK1 has been observed in various tumors, including glioblastoma, melanoma, osteosarcoma, and hepatocellular carcinoma. This protein is known to enhance actin-myosin contractility by phosphorylating regulatory myosin light chains (MLCs). It also plays a role in cell motility through the modulation of integrin β1-activated focal adhesion kinase (FAK) signaling. [3]

포장

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-601M when ordering the 250 µg size.

품질

routinely evaluated by phosphorylation of Long S6 substrate peptide (Catalog #12-420)

물리적 형태

Ni²⁺/NTA-agarose

저장 및 안정성

6 months at -70°C

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable