The SMC Phospho-alpha-Synuclein kit (03-0188-00) was tested for reactivity in 4 samples from each of the following species: Mouse, Rat, Feline, Canine, and Non-Human Primate. Phospho alpha-Synuclein was detected in all 4 samples from rats, cats, and non-human primates. It was detected in 1 out of 4 samples from canines and mice. However, the kit was not tested with standards from each species to determine the amount of cross-reactivity, but there appears to be some level of cross-reactivity.
03-0188-00
SMC® Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit
동의어(들):
p-α-synuclein S129 immunoassay kit, p-α-synuclein Ser129 immunoassay kit, p-α-synuclein Single Molecule Counting Kit, p-α-synuclein ultra-sensitive immunoassay
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모든 사진(1)
About This Item
UNSPSC 코드:
12161503
NACRES:
NA.84
가격 및 재고 정보를 현재 이용할 수 없음
추천 제품
종 반응성
mouse, monkey, cat, rat
제조업체/상표
SMC®
assay range
sensitivity: 0.1 pg/mL
(Lower Limit of Quantification)
inter-assay cv: 7%
intra-assay cv: 6%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)
기술
single molecule counting: suitable
저장 온도
2-8°C
일반 설명
The SMC® Human Phospho-a-Synuclein (S129) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
특이성
Species Reactivity:
Specificity to the following species analytes were tested with the following results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 25%
Beagle – 25%
Cross Reactivity:
No cross-reactivity was observed to an alpha-synuclein recombinant protein purified from E. Coli (i.e. non-phosphorylated alpha-synuclein).
Accuracy:
CSF Recovery: 5 CSF samples spiked with average recovery of 100% and a range of 95-103%
Serum/Plasma Recovery: 2 serum samples and 3 plasma samples with average recovery of 87% and a range of 83-91%
Specificity to the following species analytes were tested with the following results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 25%
Beagle – 25%
Cross Reactivity:
No cross-reactivity was observed to an alpha-synuclein recombinant protein purified from E. Coli (i.e. non-phosphorylated alpha-synuclein).
Accuracy:
CSF Recovery: 5 CSF samples spiked with average recovery of 100% and a range of 95-103%
Serum/Plasma Recovery: 2 serum samples and 3 plasma samples with average recovery of 87% and a range of 83-91%
애플리케이션
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human phospho-a-synuclein (S129) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of phospho-a-synuclein (S129) in human CSF, serum, and plasma.
This new streamline kit has the following features including:
Research Category:
Neuroscience, neurological
Research Sub-Category:
This new streamline kit has the following features including:
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Research Category:
Neuroscience, neurological
Research Sub-Category:
- Neurodegenerative Diseases
- Neuronal & Glial Markers
- Neuroscience
- Nervous System
포장
96 well assay
성분
- Assay Buffer
- Phospho-a-synuclein (S129) Coated Beads
- Standard Diluent
- Phospho-a-synuclein (S129) Detection Antibody
- Phospho-a-synuclein (S129) Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
법적 정보
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
관련 콘텐츠
Discover how to expand neuroscience biomarker research with high sensitivity immunoassays, such as Single Molecule Counting (SMC®) assays. These assays give researchers the tools they need to detect low-abundant blood-based biomarkers and expand biomarker options in neuroscience research.
Explore high-sensitivity SMC® neurology immunoassays that precisely measure low levels of neurology protein analytes, such as NF-L or Tau proteins.
신경면역학과 신경염증 사이토카인 및 신경변성 질환 바이오마커를 연구하기 위한 면역분석을 찾아보십시오.
Find immunoassays to explore neuroimmunology and neuroinflammation cytokines and neurodegenerative disease biomarkers.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
