LC/MS/MS Analysis of Aflatoxins in Cannabis on Ascentis^® Express 2.7 μm Phenyl-Hexyl after Cleanup using Supel^™ Tox AflaZea SPE

sample preparation

Solid Phase Extraction

sample/matrix

cannabis extract spiked with aflatoxins at corresponding concentrations of 6 ppb each (for Aflatoxin B2 and G2) and 24 ppb each (for Afltoxin B1 and G1) in cannabis

SPE tube/cartridge

Supel Tox AflaZea SPE, 6 mL (55314-U)

sample addition

2 mL of sample extract

elution

apply strong vacuum and collect the sample into plastic tube

eluate post-treatment

dilute 0.2 mL of the extract with 0.8 mL distilled water and mix well

column

Ascentis Express Phenyl-Hexyl, 5 cm x 2.1 mm I.D., 2.7 μm particles (53334-U)

mobile phase

[A] 5 mM ammonium formate with 1% formic acid in water; [B] 5 mM ammonium formate with 1% formic acid in methanol

gradient

30% B to 60% B in 3 min; to 100% B in 2 in; held at 100% B for 2 min; to 30% B in 0.1 min; held at 30% B for 1.4 min

flow rate

0.4 mL/min

pressure

5511 psi (380 bar)

column temp.

40 °C

detector

MS, ESI(+), MRM 331.3/189.0, 329.1/243.0, 315.9/259.0, 313.1/241.0

injection

10 μL