HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18
CONDITIONS
column
Ascentis® Express C18, 25 cm x 4.6 mm I.D., 5 μm particles (50538-U)
mobile phase
[A] acetonitrile; [B] methanol; (95:5, A:B)
flow rate
1.5 mL/min
pressure
1726 psi (119 bar)
column temp.
35 °C
detector
UV, 265 nm
injection
10 μL
sample
50 μg/mL in 90:10, acetonitrile:methanol
詳細
アナリシスノート
This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany