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HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl, Methanol Mobile Phase

HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl, Methanol Mobile Phase application for HPLC

材料

Standard

製品番号
詳細
価格

D7282

デスメチルジアゼパム

analytical standard

O-902

オキサゼパム 溶液

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

A-903

アルプラゾラム 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-907

クロナゼパム 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

D-907

ジアゼパム 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

分析カラム

製品番号
詳細
価格

53348-U

Ascentis® Express フェニルヘキシル、2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)

mobile phase

[A] water; [B] methanol; (35:65, A:B)

flow rate

1.4 mL/min

pressure

3600 psi (248 bar)

column temp.

35 °C

detector

UV, 250 nm

injection

2 μL

sample

0.1 g/L in 20% methanol

詳細

アナリシスノート

The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Applications

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