HPLCによる3,4-ジヒドロキシフェニルアラニン (DOPA)エナンチオマーの分析、CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] acetonitrile; (25:75, A:B)

flow rate

0.9 mL/min

column temp.

25 °C

detector

UV, 220 nm

injection

5 μL

sample

2 mg/mL in methanol