GCによるピーナツバター中のピラジン分析、 SUPELCOWAX 10、SPME処理、50/30 μm DVB/Carboxen/PDMS使用
材料
SPMEファイバー
SPMEファイバーアセンブリー ジビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS)
needle size 24 ga, for use with manual holder分析カラム
標準
CONDITIONS
sample/matrix
5 g peanut butter in 40 mL vial
SPME fiber
divinylbenzene-Carboxen-polydimethylbenzene StableFlex (57328-U)
extraction
headspace, 30 min at 65 °C in heating block
desorption process
270 °C for 5 min
column
SUPELCOWAX 10, 30 m × 0.25 mm, 0.25 μm (24079)
oven
40 °C (5 min) to 230 °C at 4 °C/min
inj. temp.
270 °C
injection
splitless/split, closed 0.5 min, 270 °C, with 0.75 mm liner
detector
ion trap mass spectrometer, m/z =30-350 at 0.6 sec/scan. Selected ions used for quantitation.
carrier gas
Helium, 30 cm/sec
liner
0.75 mm
sample
peanut butter
詳細
アナリシスノート
In this application, SPME was used for the analysis of volatiles in multifloral honey using a headspace method. Headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.
法的情報
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