コンテンツへスキップ
Merck
  • Isolation and drug susceptibility of Candida parapsilosis sensu lato and other species of C. parapsilosis complex from patients with blood stream infections and proposal of a novel LAMP identification method for the species.

Isolation and drug susceptibility of Candida parapsilosis sensu lato and other species of C. parapsilosis complex from patients with blood stream infections and proposal of a novel LAMP identification method for the species.

Mycopathologia (2014-12-08)
Plinio Trabasso, Tetsuhiro Matsuzawa, Renata Fagnani, Yasunori Muraosa, Kenichiro Tominaga, Mariangela Ribeiro Resende, Katsuhiko Kamei, Yuzuru Mikami, Angelica Zaninelli Schreiber, Maria Luiza Moretti
要旨

Candida parapsilosis complex (CPC) is the third Candida species isolated in blood cultures of patients from our Hospital, following C. albicans and C. tropicalis. From 2006 to 2010, the median annual distribution of CPC was 8 cases/year. Records of 36 patients were reviewed. CPC were 31 (86.1%) C. parapsilosis; 4 (11.1%) C. orthopsilosis; and 1 (2.8%) C. metapsilosis. Clinical characteristics were central venous catheter, 34 (94.4%); parental nutrition, 25 (70%); surgery, 27 (57.9%); prior bacteremia, 20 (51.3%); malignancy, 18 (50%). General mortality was 47.2%. Death was higher in immunosuppressed patients (17 vs. 11; p = 0.003). Three out four (75%) patients with C. orthopsilosis and 14 out 31 (45.2%) with C. parapsilosis died (p = 0.558). Thirty-nine individual isolates were tested for susceptibility to seven antifungal drugs, with MICs values showing susceptibility to all of them. Two isolates, one C. orthopsilosis and one C. parapsilosis, had fluconazole MIC = 4 μg/mL. Differentiation among CPC has implication in caring for patients with invasive candidiasis since there are differences in virulence, pathogenicity and drug susceptibility. A method targeting the topoisomerase II gene based on loop-mediated isothermal amplification (LAMP) was developed. LAMP emerges as a promising tool for the identification of fungal species due to the high sensitivity and specificity. LAMP can be performed at the point-of-care, being no necessary the use of expensive equipment. In our study, the method was successful comparing to the DNA sequencing and proved to be a reliable and fast assay to distinguish the three species of CPC.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
エチルアルコール(純粋), for molecular biology
Sigma-Aldrich
エタノール, JIS special grade, ≥99.5%
Sigma-Aldrich
エタノール, SAJ first grade, ≥99.5%
Sigma-Aldrich
フルコナゾール, ≥98% (HPLC), powder
Sigma-Aldrich
イトラコナゾール, ≥98% (HPLC)
Sigma-Aldrich
エチルアルコール(純粋), 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
エタノール, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Sigma-Aldrich
5-フルオロシトシン, nucleoside analog
Supelco
エタノール 溶液, certified reference material, 2000 μg/mL in methanol
Sigma-Aldrich
エタノール, puriss. p.a., absolute, ≥99.8% (GC)
Supelco
エタノール標準品10% (v/v), 10 % (v/v) in H2O, analytical standard
USP
フルコナゾール, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
エタノール, ≥99.5%, suitable for HPLC
Sigma-Aldrich
ボリコナゾール, ≥98% (HPLC)
Sigma-Aldrich
エタノール, JIS special grade, 94.8-95.8%
Sigma-Aldrich
エタノール, ≥99.5%
Sigma-Aldrich
エタノール, ≥99.5%, suitable for absorption spectrum analysis
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sigma-Aldrich
エタノール, 94.8-95.8%
USP
Miconazole, United States Pharmacopeia (USP) Reference Standard
Supelco
フルコナゾール, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
エタノール, ≥99.5%, SAJ super special grade
Supelco
ボリコナゾール, VETRANAL®, analytical standard
Sigma-Aldrich
エタノール, JIS 1000, ≥99.5%, for residue analysis
Sigma-Aldrich
エタノール, ≥99.5%, suitable for fluorescence
Sigma-Aldrich
エタノール, JIS 300, ≥99.5%, for residue analysis
ボリコナゾール, European Pharmacopoeia (EP) Reference Standard
フルコナゾール, European Pharmacopoeia (EP) Reference Standard
イトラコナゾール, European Pharmacopoeia (EP) Reference Standard
Miconazole, European Pharmacopoeia (EP) Reference Standard