コンテンツへスキップ
Merck
  • Layer-by-layer immobilized catalase on electrospun nanofibrous mats protects against oxidative stress induced by hydrogen peroxide.

Layer-by-layer immobilized catalase on electrospun nanofibrous mats protects against oxidative stress induced by hydrogen peroxide.

Journal of biomedical nanotechnology (2014-05-09)
Rong Huang, Hongbing Deng, Tongjian Cai, Yingfei Zhan, Xiankai Wang, Xuanxuan Chen, Ailing Ji, Xueyong Lil
要旨

Catalase, a kind of redox enzyme and generally recognized as an efficient agent for protecting cells against hydrogen peroxide (H2O2)-induced cytotoxicity. The immobilization of catalase was accomplished by depositing the positively charged chitosan and the negatively charged catalase on electrospun cellulose nanofibrous mats through electrospining and layer-by-layer (LBL) techniques. The morphology obtained from Field emission scanning electron microscopy (FE-SEM) indicated that more orderly arranged three-dimension (3D) structure and roughness formed with increasing the number of coating bilayers. Besides, the enzyme-immobilized nanofibrous mats were found with high enzyme loading and activity, moreover, X-ray photoelectron spectroscopy (XPS) results further demonstrated the successful immobilization of chitosan and catalase on cellulose nanofibers support. Furthermore, we evaluated the cytotoxicity induced by hydrogen peroxide in the Human umbilical vascular endothelial cells with or without pretreatment of nanofibrous mats by MTT assay, LDH activity and Flow cytometric evaluation, and confirmed the pronounced hydrogen peroxide-induced toxicity, but pretreatment of immobilized catalase reduced the cytotoxicity and protected cells against hydrogen peroxide-induced cytotoxic effects which were further demonstrated by scanning electron microscopy (SEM) and Transmission Electron Microscopy (TEM) images. The data pointed toward a role of catalase-immobilized nanofibrous mats in protecting cells against hydrogen peroxide-induced cellular damage and their potential application in biomedical field.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
キトサン, low molecular weight
Sigma-Aldrich
キトサン, medium molecular weight
Sigma-Aldrich
セルロース, microcrystalline, powder, 20 μm
Sigma-Aldrich
キトサン, high molecular weight
Sigma-Aldrich
カタラーゼ ウシ肝臓由来, lyophilized powder, 2,000-5,000 units/mg protein
Sigma-Aldrich
カタラーゼ ウシ肝臓由来, lyophilized powder, ≥10,000 units/mg protein
Sigma-Aldrich
セルロース, microcrystalline, powder
Sigma-Aldrich
カタラーゼ ウシ肝臓由来, powder, suitable for cell culture, 2,000-5,000 units/mg protein
Sigma-Aldrich
キトサン, from shrimp shells, ≥75% (deacetylated)
Sigma-Aldrich
L-乳酸デヒドロゲナーゼ ウサギ筋肉由来, Type XI, lyophilized powder, 600-1,200 units/mg protein
Sigma-Aldrich
セルロース, fibers, (medium)
Sigma-Aldrich
過酸化水素 溶液, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O
Sigma-Aldrich
過酸化水素 溶液, purum p.a., ≥35% (RT)
Sigma-Aldrich
キトサン, from shrimp shells, practical grade
Sigma-Aldrich
L-乳酸デヒドロゲナーゼ ウサギ筋肉由来, Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
Sigma-Aldrich
Sigmacell セルロース, Type 20, 20 μm
Sigma-Aldrich
α-セルロース, powder
Sigma-Aldrich
カタラーゼ ウシ肝臓由来, aqueous suspension, 10,000-40,000 units/mg protein
Sigma-Aldrich
カタラーゼ ウシ肝臓由来, aqueous suspension, 40,000-60,000 units/mg protein (E1%/405)
Sigma-Aldrich
セルロース, colloidal, microcrystalline
Sigma-Aldrich
L-乳酸デヒドロゲナーゼ ウシ心臓由来, Type III, ammonium sulfate suspension, ≥500 units/mg protein
Sigma-Aldrich
L-乳酸デヒドロゲナーゼ ウシ心臓由来, 1000 units/mL
Sigma-Aldrich
カタラーゼ ウシ肝臓由来, aqueous solution, ≥30,000 units/mg protein
Sigma-Aldrich
キトサン エビ殻由来, low-viscous
Sigma-Aldrich
過酸化水素 溶液, JIS special grade, 30.0-35.5%
Sigma-Aldrich
カタラーゼ from Aspergillus niger, ammonium sulfate suspension, ≥4,000 units/mg protein
Millipore
過酸化水素 溶液, 3%, suitable for microbiology
Sigma-Aldrich
過酸化水素 溶液, SAJ first grade, ≥30.0%
Sigma-Aldrich
Sigmacellセルロース, Type 101, Highly purified, fibers
Sigma-Aldrich
Sigmacell セルロース, Type 50, 50 μm