Analysis of volatiles from Spanish honeys by solid-phase microextraction and gas chromatography-mass spectrometry.

Analysis of volatiles from Spanish honeys by solid-phase microextraction and gas chromatography-mass spectrometry.

Journal of agricultural and food chemistry (2002-04-18)

Rosa A Pérez, Consuelo Sánchez-Brunete, Rosa M Calvo, José L Tadeo

PMID11958634

要旨

Headspace solid-phase microextraction (SPME), followed by gas chromatography (GC)-mass spectrometry (MS) determination, has been used for the analysis of honey volatiles. Two SPME fibers were employed to study the composition of volatiles from various types of Spanish honeys. The best results were obtained with the Carboxen/PDMS fiber, using a homogenization time of 1 h at 70 degrees C and a sampling period of 30 min. A total of 35 compounds were detected, most of them identified by GC-MS and quantified using external standards. Differences in the composition of honey volatiles were obtained, and these results allowed the differentiation of honeys. However, further studies are necessary to confirm the utility of this technique as an alternative tool for the characterization of the floral origin of honeys.

材料

製品番号

ブランド

製品内容

57348-U

Supelco

SPMEファイバーアセンブリージビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS), needle size 24 ga, StableFlex

57298-U

Supelco

SPMEファイバーアセンブリージビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler

57328-U

Supelco

SPMEファイバーアセンブリージビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder

20358

Supelco

ORBO^™ Carboxen-564チューブ, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

57299-U

Supelco

SPMEファイバーアセンブリージビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm

57329-U

Supelco

SPMEファイバーアセンブリージビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler

20355

Supelco

Carboxen^® 564特殊洗浄済み（20/45）、400/200 mg入りORBO^™ 78 HBR, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

57318

Supelco

SPMEファイバー・アセンブリカルボキセン／ポリジメチルシロキサン（CAR/PDMS）, d_f 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

25467

Supelco

Carboxen^®-1010 PLOTキャピラリーGCカラム, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

10478-U

Supelco

Carboxen^®吸着剤, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

25461

Supelco

Carboxen^®-1006 PLOTキャピラリーGCカラム, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57335-U

Supelco

SPMEファイバー・アセンブリカルボキセン／ポリジメチルシロキサン（CAR/PDMS）, d_f 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler

57295-U

Supelco

SPMEファイバー・アセンブリカルボキセン／ポリジメチルシロキサン（CAR/PDMS）, d_f 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler

57319

Supelco

SPMEファイバー・アセンブリカルボキセン／ポリジメチルシロキサン（CAR/PDMS）, d_f 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

24246

Supelco

Carboxen^®-1010 PLOTキャピラリーGCカラム, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

57334-U

Supelco

SPMEファイバー・アセンブリカルボキセン／ポリジメチルシロキサン（CAR/PDMS）, d_f 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber

57343-U

Supelco

SPMEファイバー・アセンブリカルボキセン／ポリジメチルシロキサン（CAR/PDMS）, d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler

57344-U

Supelco

SPMEファイバー・アセンブリカルボキセン／ポリジメチルシロキサン（CAR/PDMS）, d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder

24241-U

Supelco

Carboxen^®-1006 PLOTキャピラリーGCカラム, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

11072-U

Supelco

Carboxen^®吸着剤, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

54041-U

Supelco

Carboxen^® 1000リバーシブルSPEチューブ, 200 mg/mL, pk 30

57914-U

Supelco

SPMEファイバーアセンブリージビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm

10477-U

Supelco

Carboxen^®吸着剤, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

28718-U

Supelco

Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

10269

Supelco

Carboxen^®吸着剤, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

10471

Supelco

Carboxen^® 1003, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

23446-U

Supelco

Carboxen^® 1000, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea

57912-U

Supelco

SPMEファイバーアセンブリージビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 1 cm

11048-U

Supelco

Carboxen^®吸着剤, matrix Carboxen^® 569, 20-45 mesh, bottle of 500 g

54294-U

Supelco

カルボキセン^® 572 for Tobacco Smoke, Adsorbent Bed Wt: 300 mg, cartridge size 3 mL, pkg of 50 ea