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製品名
Ascentis® Express F5、2.7 μm HPLCカラム, 2.7 μm particle size, L × I.D. 2 cm × 2.1 mm
物質
stainless steel column
品質水準
認証
suitable for USP L43
製品種目
Ascentis®
特徴
endcapped
メーカー／製品名
Ascentis®
包装
1 ea of
パラメーター
60 °C temp. range
600 bar max. pressure (9000 psi)
テクニック
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × 内径
2 cm × 2.1 mm
表面積
135 m2/g
不純物
<5 ppm metals
Matrix
Fused-Core particle platform
superficially porous particle
マトリックス活性基
PFP (pentafluorophenyl) phase
粒径
2.7 μm
ポアサイズ
90 Å pore size
使用pH
2-8
アプリケーション
food and beverages
分離法
reversed phase
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関連するカテゴリー
詳細
Ascentis Express F5のペンタフルオロフェニルプロピル固定相は、電気陰性フッ素の存在のため電子不足フェニル環を持つ安定な逆相充填剤となります。pi-pi相互作用とマイルドな立体的相互作用の形成に加え、F5固定相は極性相互作用により化合物を保持します。Ascentis Express F5は、C18由来の交互選択性により塩基性、酸性、または中性の化合物で使用できます。
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
ガードカートリッジ
必要ですが提供されていません
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
資料
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
プロトコル
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
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What is the Department of Transportation shipping information for this product?
1 回答-
役に立ちましたか？
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Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
1 回答-
Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
役に立ちましたか？
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Can Ascentis® Express F5 HPLC columns be used for LC-MS?
1 回答-
Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
役に立ちましたか？
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Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
1 回答-
Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
役に立ちましたか？
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What flow rate should I use with Ascentis® Express F5 HPLC columns?
1 回答-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis® Express F5 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
役に立ちましたか？
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What is the carbon load for Ascentis® Express F5 HPLC Columns?
1 回答-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
役に立ちましたか？
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Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
1 回答-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
役に立ちましたか？
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 回答-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
役に立ちましたか？
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What is the pressure rating for Ascentis® Express F5 HPLC Columns?
1 回答-
Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
役に立ちましたか？
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Are guard columns available for Ascentis® Express F5 2.1 mm ID HPLC Columns?
1 回答-
Yes, we have guard columns available for Ascentis® Express F5 HPLC Column . Product 53500-U is the Ascentis® Express Guard Holder and Product 53594-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 2.1 mm .
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