W4769

モノクローナル抗WIPI-1抗体 マウス宿主抗体

~1.5 mg/mL, clone WIPI1-4, purified immunoglobulin, buffered aqueous solution

• 別名: 抗ATG18抗体, 抗WIPI-1α抗体, 抗WIPI49抗体
• UNSPSCコード: 12352203

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: WIPI1-4, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~49 kDa
• 交差性: human, mouse, bovine, rat
• 濃度: ~1.5 mg/mL
• テクニック: western blot: 2-4 μg/mL using whole extract of G361 cells
• UniProtアクセッション番号: Q5MNZ9
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... WIPI1(55062) ; mouse ... Wipi1(52639) ; rat ... Wipi1(303630)

詳細

詳細

Monoclonal Anti-WIPI-1 (mouse IgG1 isotype) is derived from the hybridoma WIPI1-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human WIPI-1, conjugated to KLH. WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) the mammalian orthologue of Atg18 in S. cerevisiae and A. thaliana, is a member of the WIPI subfamily of WD-repeat (Trp-Asp) proteins. WIPI-1 has a 7-bladed propeller structure and contains a conserved motif for interaction with phospholipids. WIPI-1 is expressed in normal human tissues with high levels in skeletal muscle, heart and testis.

アプリケーション

Monoclonal Anti-WIPI-1 antibody has been used in immunoblotting, immunoprecipitation.

生物化学的／生理学的作用

WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) plays an important role in signal transduction, transcription regulation and apoptosis. Endogenous WIPI-1 partially colocalizes with the autophagosomal marker LC3 and induction of autophagy leads to the accumulation of WIPI-1 in large vesicular and cup-shaped structures that are characteristic for autophagy-linked proteins. WIPI-1 is aberrantly expressed in human cancer and is linked to starvation induced autophagy.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.