おすすめの製品
由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
195C, monoclonal
フォーム
buffered aqueous solution
分子量
antigen ~167 kDa
化学種の反応性
human
濃度
~1 mg/mL
テクニック
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... WRN(7486)
詳細
Anti-WRN antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 195C produced by the fusion of mouse myeloma cells (p3-NS1/Ag4-1) and splenocytes from BALB/c mice immunized with a recombinant fusion protein fragment of human WRN. Werner syndrome ATP-dependent helicase (WRN) is located on chromosome 8 and is 167 kDa. The WRN protein belongs to the RecQ helicase family of DNA helicases.
免疫原
ヒトWRN (アミノ酸1074~1432) のリコンビナント融合タンパク質。
アプリケーション
Anti-WRN antibody has been used in
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunohistochemistry
- western blotting
生物化学的／生理学的作用
Werner syndrome ATP-dependent helicase (WRN) protein has been demonstrated to possess three known catalytic activities: 3′-5′ helicase, exonuclease and ATPase activities. The C-terminal region of the protein contains a nuclear localization signal (NLS), thus targeting WRN protein to the nucleus, nucleolus and nucleoplasmic foci. The involvement of WRN in multiple DNA metabolic process suggests its function as a tumor suppressor. Mutations in the WRN protein results in Werner syndrome (WS). The epigenetic inactivation of WRN leads to loss of WRN-exonuclease activity, resulting in increased chromosomal instability and hypersensitivity to chemotherapeutic drugs.
物理的形状
0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
W0393-200UL:
W0393-25UL:
W0393-VAR:
W0393-BULK:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Bidisha Saha et al.
Molecular genetics & genomic medicine, 1(1), 7-14 (2013-08-13)
Werner syndrome is a rare autosomal recessive disorder characterized by multiple features consistent with accelerated aging. It is caused by mutations in the WRN gene, which encodes a RecQ type helicase. To date, more than 70 disease-causing mutations have been
Yuyao Tian et al.
Cell & bioscience, 14(1), 7-7 (2024-01-07)
Metabolic dysfunction is one of the main symptoms of Werner syndrome (WS); however, the underlying mechanisms remain unclear. Here, we report that loss of WRN accelerates adipogenesis at an early stage both in vitro (stem cells) and in vivo (zebrafish).
Class I histone deacetylase HDAC1 and WRN RECQ helicase contribute additively to protect replication forks upon hydroxyurea-induced arrest
Kehrli K, et al.
The Journal of Biological Chemistry, 291(47), 24487-24503 (2016)
S Deepak Amalnath et al.
Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology, 36(4), 323-325 (2017-08-11)
Werner syndrome is a rare progeroid syndrome caused by the WRN gene mutation. It is characterized by a general appearance of premature aging, diabetes mellitus, and atherosclerosis, and an increased risk of malignancies. We report a patient who presented with
Linda J W Bosch et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(18), 4612-4622 (2016-04-29)
WRN promoter CpG island hypermethylation in colorectal cancer has been reported to increase sensitivity to irinotecan-based therapies. We aimed to characterize methylation of the WRN promoter, determine the effect of WRN promoter hypermethylation upon expression, and validate a previous report
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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