SRP5172

Catenin β、GSTタグ融合 ヒト

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 別名: CTNNB, FLJ25606
• CAS番号: 847964-78-7
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: ≥70% (SDS-PAGE)
• フォーム: buffered aqueous glycerol solution
• 分子量: ~115 kDa
• テクニック: activity assay: suitable
• 溶解性: water: soluble
• 適合性: suitable for molecular biology
• NCBIアクセッション番号: NM_001904
• アプリケーション: cell analysis
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... CTNNB1(1499)

詳細

詳細

Research area: Apoptosis. β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins are associated with E-cadherin at cellular junctions.[1] CTNNB1 (catenin β-1) belongs to the armadillo family of proteins. This protein possesses a 130 amino acid N-terminal domain, 12 imperfect repeats of 42 amino acids termed arm repeats, and a 100 amino acid C-terminal domain. [2] The gene encoding β-catenin is localized on human chromosome 3p22.1.[3]

アプリケーション

Catenin β, GST tagged human has been used:

• as a component of protein kinase buffer for in vitro kinase assay[4]
• in in vitro citrullination assay to characterize β-catenin citrullination[5]
• to perform differentiation factor screening[6]

生物化学的／生理学的作用

β-catenin interacts with T-cell factor and lymphoid enhancer-binding factor (TCF and LEF) transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway.[1] The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and glycogen synthase kinase 3 β (GSK3β), form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of βcatenin by the proteasome.[2] APC and the Siah-1 E3 ubiquitin-protein ligase-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas.[7][8] The protein has a role in homeostasis and development.[2]

物理的形状

50 mM Tris-HCl(pH 7.5, 150 mM NaCl, 10 mMグルタチオン, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25%グリセロール含有)を用いた溶液として調製されています。

調製ノート

開封後はそれぞれの目的に応じて少量ずつ分注し、-70°Cで保存してください。また、取扱いや凍結融解について、繰り返しのご使用は避けてください。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable