SRP4902

アディポネクチン マウス由来

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 別名: ACDC, APM-1, Acrp30, AdipoQ, GBP-28
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: mouse
• リコンビナント: expressed in E. coli
• アッセイ: ≥90% (SDS-PAGE)
• 形状: lyophilized
• 分子量: ~30 kDa
• 包装: pkg of 25 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 不純物: endotoxin, tested
• NCBIアクセッション番号: Q60994
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: mouse ... Adipoq(11450)

詳細

詳細

Acrp30 (adipocyte complement-related protein), or cytokine adiponectin, is a hormone produced and secreted by differentiated adipocytes. This protein is composed of an N-terminal collagenous domain and a C-terminal globular trimerization domain. It exists in plasma predominantly as trimeric, hexameric or other high-molecular weight forms. The globular subunit gAcrp30 is however absent in plasma and is biologically active. gAcrp30 is the truncated form of full length of Acrp30, and functions as a ligand of AdipoR1.

アプリケーション

Adiponectin from mouse has been used for the treatment of 3T3-L1 cells, along with TNFα (tumor necrosis factor), to determine TNFα inhibition of adiponectin activities by examining lipid accumulation and glucose uptake. It has also been used for the pretreatment of TM3 cells, to study its influence on attenuation of proinflammatory cytokine expression in TM3 cells upon treatment with TNF-α or IL-1β (interleukin).

生物化学的／生理学的作用

In obese mice and humans, adiponectin mRNA expression is reduced, therefore linking this hormone to energy homeostasis. In incubated mouse muscle and cultured cells, gAcrp30 leads to an increase in fatty acid oxidation. This hormone is also linked to anti-atherogenesis, and in apoE-deficient mice, overexpression of gAcrp30 results in significant attenuation of atherosclerosis. Long-term administration of this hormone in mice results in weight loss without reduction in food intake. This hormone is capable of reducing proliferation of MDA-ERα7 cell line. Studies show that ratio of gAcrp30 to leptin and the ERα(estrogen receptor alpha) status of cells are key in determining BRCA (breast cancer) cell growth.

物理的形状

凍結乾燥品(25 mM Tris溶液, pH 7.4, 0.15 M NaCl, アディポネクチン1 μgあたりBSA 50 μg含有, 0.2 μmフィルター滅菌済み)。

再構成

PBS(BSAを0.1%以上含有)で濃度0.1～1.0 mg/mLに再構成してください。このストック溶液は、他の水性バッファーで希釈することができ、4°Cで1週間、-20°Cではさらに長期間保存することができます。

バイアルは、開封前に遠心してください。凍結融解の繰り返しは避けてください。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable