SRP3133

OX40 リガンド ヒト

recombinant, expressed in Hi-5 Insect cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

• 別名: CD252, Gp34, OX40L, TNFSF4
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in Hi-5 Insect cells
• アッセイ: ≥97% (HPLC); ≥97% (SDS-PAGE)
• フォーム: lyophilized
• 分子量: 15.5-25.0 kDa
• 包装: pkg of 10 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.1 EU/μg endotoxin, tested
• 色: white to off-white
• UniProtアクセッション番号: P23510
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... TNFSF4(7292)

詳細

詳細

OX40L or tumor necrosis factor superfamily member 4 (TNFSF4), which is a member of the TNF superfamily of structurally related proteins, exists primarily as a type II membrane bound, non-covalently linked homotrimeric protein. This cytokine is expressed on antigen presenting cells (APCs),[1] such as dendritic cells and activated B-cells, and also on various other cells such as vascular endothelial cells, mast cells, and natural killer cells.[2][3] It has a 23 amino acid cytoplasmic tail and a 133 amino acid extracellular domain. The gene encoding this protein is localized on human chromosome 1q25 and has three exons.[1] Recombinant OX40L is a glycosylated 133 amino acid protein corresponding to the extracellular TNF homologous domain of the full length transmembrane protein. It migrates with an apparent molecular mass of 15.5-25kDa on SDS-PAGE.

生物化学的／生理学的作用

OX40L signals specifically through the OX40 receptor, which is expressed predominantly on CD4⁺T cells but also on certain activated CD8⁺T cells. OX40/OX40L functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells.[1] It enhances cell proliferation and survival, and increases expression of regulated on activation, normal T cell expressed and secreted (RANTES) or chemokine ligand 5 (CCL5), interleukins-2 and -3, and interferon-γ (IFNγ).[2] OX40/OX40L signaling plays an important role in immuno-regulatory communication and immune-tolerance. Polymorphisms in the TNFSF4 gene have been associated with autoimmune thyroid diseases (AITDs), rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc).[1]

シーケンス

QVSHRYPRIQ SIKVQFTEYK KEKGFILTSQ KEDEIMKVQN NSVIINCDGF YLISLKGYFS QEVNISLHYQ KDEEPLFQLK KVRSVNSLMV ASLTYKDKVY LNVTTDNTSL DDFHVNGGEL ILIHQNPGEF CVL

物理的形状

凍結乾燥品(10 mM Tris, pH 8.5)。

再構成

バイアルは、開封前に遠心してください。水で濃度0.1～1.0 mg/mLに復元してください。ボルテックスは行なわないでください。水溶液は2～8°Cで最長1週間保存できます。長期保存には、担体タンパク質(0.1% BSAなど)含有バッファーでさらに希釈し、1回の使用量ずつに分注して-20～-80°Cで保存することを推奨します。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable