SRP0736

PP2A ALPHA/PPP2R1A Complex, Active

recombinant

• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: 70-95% (SDS-PAGE)
• 形状: frozen liquid
• 比活性: 3400-4600 nmol/min·mg (BIOMOL Green)
• 分子量: ~38 kDa; ~65 kDa
• 濃度: 0.1 μg/μL
• アクセッション番号: NM_002715; NM_014225
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PP2A(5524) , PPP2R1A(5518)

詳細

アプリケーション

Useful for Phosphatase assay and Western blot.

生物化学的／生理学的作用

Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A ⁽¹⁾. Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity ⁽²⁾.

再構成

Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

保管および安定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H360D
• 注意書き: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable