SRE0021

グランザイムB ヒト

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

• 別名: SECT, T細胞セリンプロテアーゼ1-3E, グランザイム-2, グランザイムB, リンパ球プロテアーゼ, 細胞傷害性Tリンパ球プロテイナーゼ2
• UNSPSCコード: 12352204
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in insect cells
• アッセイ: ≥90% (SDS-PAGE)
• 形状: lyophilized powder
• 有効性: >0.004 units/mg
• 比活性: ≥40 units/μg
• 包装: pkg of 5 μg
• UniProtアクセッション番号: P10144
• 輸送温度: wet ice
• 保管温度: −70°C
• 遺伝子情報: human ... GZMB(3002)

詳細

詳細

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

生物化学的／生理学的作用

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

単位の定義

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.

物理的形状

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable